Cultures in Vitro of Blood Cells, Bone Marrow, and Myocardium From Leukotic Fowls*

A thorough description of this strain has been given by Engelbreth-Holm and his associates (5, 16). Strain T originated from a spontaneous case of anemic hemocytoblastosis. After the 5th passage the strain divided into 2 branches. One which was almost purely myeloblastic died out after I6 passages. The other, a purely hemocytoblastic branch gives practically ioo per cent takes. The disease usually runs an acute course, but in a small percentage of cases it takes the form of a chronic erythroblastic anemia. Both types are essentially the same. According to Uhl, Engelbreth-Holm, and Rothe Meyer (i6) intramuscular and subcutaneous inoculation of leukotic material often causes sarcoma at the site of inoculation, which in most cases is accompanied by hemocytoblastosis.